منابع مشابه
Novobiocin and coumermycin inhibit DNA supercoiling catalyzed by DNA gyrase.
Novobiocin and coumermycin are known to inhibit the replication of DNA iing of DNA catalyzed by E. coli DNA gyrase, a recently discovered enzyme that introduces negative superhelical turns into covalently circular DNA. The activity of DNA gyrase purified from a coumermycin-resistant mutant strain is resistant to both drugs. The inhibition by novobiocin of colicin E1 plasmid DNA replication in a...
متن کاملGyrase inhibitors and intracellular DNA supercoiling.
cells are known to require ATP for all catalytic activities. However, during the purification of the enzymes, some of their native properties may be lost due to proteolytic degradation or to a resolution of their supramolecular structure (Glikin et al., 1984). The trypanosomal extracts, as well as the enzyme obtained after hydroxylapatite chromatography, are capable of catenating supercoiled ci...
متن کاملNucleotide- and stoichiometry-dependent DNA supercoiling by reverse gyrase.
Reverse gyrase is a unique type IA topoisomerase that can introduce positive supercoils into DNA. We have investigated some of the biochemical properties of Archaeoglobus fulgidus reverse gyrase. It can mediate three distinct supercoiling reactions depending on the adenine nucleotide cofactor that is present in the reaction. Besides the ATP-driven positive supercoiling reaction, the enzyme can ...
متن کاملStructural insight into negative DNA supercoiling by DNA gyrase, a bacterial type 2A DNA topoisomerase
Type 2A DNA topoisomerases (Topo2A) remodel DNA topology during replication, transcription and chromosome segregation. These multisubunit enzymes catalyze the transport of a double-stranded DNA through a transient break formed in another duplex. The bacterial DNA gyrase, a target for broad-spectrum antibiotics, is the sole Topo2A enzyme able to introduce negative supercoils. We reveal here for ...
متن کاملDNA gyrase with a single catalytic tyrosine can catalyze DNA supercoiling by a nicking-closing mechanism
The topological state of DNA is important for replication, recombination and transcription, and is regulated in vivo by DNA topoisomerases. Gyrase introduces negative supercoils into DNA at the expense of ATP hydrolysis. It is the accepted view that gyrase achieves supercoiling by a strand passage mechanism, in which double-stranded DNA is cleaved, and a second double-stranded segment is passed...
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ژورنال
عنوان ژورنال: Nature
سال: 1981
ISSN: 0028-0836,1476-4687
DOI: 10.1038/294607a0